| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161116 |
| Chromosome: | chromosome 4 |
| Location: | 2588281 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222600 | PHC46 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATAGGTGGCGCGGGCACGTATGATCAGC |
| Internal bar code: | TTCCGCAAGGCCTGGCGACCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 99.3316 |
| LEAP-Seq n confirming: | 743 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGAGGGCTGAGGTCACTC |
| Suggested primer 2: | TGAGTTGCAAGGATGACTCG |