Insertion junction: LMJ.RY0402.161123_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.161123
Chromosome:chromosome 1
Location:7374453
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g053000 GPD1,GPDH2,GPD2 (1 of 3) K00006 - glycerol-3-phosphate dehydrogenase (NAD+) (GPD1); Glycerol-3-phosphate dehydrogenase/dihydroxyacetone-3-phosphate reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCTGCCTCCACACACACACGCAGGCCA
Internal bar code:GACACGGTCTATCACAGGTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:28
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACTTTCTCCCCTCCCCTAC
Suggested primer 2:GCCAATCTTCTCGGTAACCA