| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161150 |
| Chromosome: | chromosome 5 |
| Location: | 322055 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233300 | (1 of 1) 2.1.1.98 - Diphthine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTACGTTCCCCTTGGTACGCAGCTCCC |
| Internal bar code: | TTCCCCAGTCACCAGACCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 99.7802 |
| LEAP-Seq n confirming: | 454 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCGTACCACACCTTGTTG |
| Suggested primer 2: | TGCATGTGTGGAATTGTGTG |