| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161170 |
| Chromosome: | chromosome 14 |
| Location: | 662550 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612300 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGAAGCCATGTATTCTATAACAACGCAG |
| Internal bar code: | AGCATTCAAACGTCTAAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 99.5838 |
| LEAP-Seq n confirming: | 7657 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGGTGATTCGCAATGAG |
| Suggested primer 2: | TTGAGGACTAGAAGCGCCAT |