| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.161214 |
| Chromosome: | chromosome 7 |
| Location: | 2087282 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g326050 | KIL8 | Putative kinesin-like motor protein; (1 of 2) PTHR13140//PTHR13140:SF410 - MYOSIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCAACCCACTCACCCCGCATGAGCGCC |
| Internal bar code: | CGGGATTGACGGGCTGCCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 99.6977 |
| LEAP-Seq n confirming: | 6595 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCGCTCGACCATAACAC |
| Suggested primer 2: | CCCAAGTCAAGGCTGTGTTT |