| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.161228 |
| Chromosome: | chromosome 6 |
| Location: | 2445807 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268650 | FAP279 | (1 of 8) PF14580 - Leucine-rich repeat (LRR_9); Flagellar Associated Protein 279 | 5'UTR|CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCGCTCTGGCTGAACGGGAATAAGCTCA |
| Internal bar code: | ACCGGGCTTCGACTTTTACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 230 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGGAAGTTGAGACAAGC |
| Suggested primer 2: | GAGCGTTAACCAGCTCATCC |