| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161236 |
| Chromosome: | chromosome 14 |
| Location: | 598085 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611950 | PPR-superfamily protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGGCTATCTTGCGACTTATCGTCCAGCA |
| Internal bar code: | TCGTTTCGAGATTCCGGATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 426 |
| LEAP-Seq percent confirming: | 99.8763 |
| LEAP-Seq n confirming: | 1615 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAGAGGTTGGTGGTGTG |
| Suggested primer 2: | TCTTGTGAAATTCAGCGCAC |