| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161256 |
| Chromosome: | chromosome 17 |
| Location: | 1209657 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g704950 | (1 of 2) PTHR13140//PTHR13140:SF410 - MYOSIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGCCTACGGTTCATGGCGACGACGGCC |
| Internal bar code: | AGGGCCGCGGAGCATAGAGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 774 |
| LEAP-Seq percent confirming: | 99.6422 |
| LEAP-Seq n confirming: | 1114 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACGTCAGCCTGAACGAG |
| Suggested primer 2: | GGAGGAGCACTAGCATCCTG |