Insertion junction: LMJ.RY0402.161260_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.161260
Chromosome:chromosome 12
Location:2367585
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g507500 (1 of 5) IPR001005//IPR009057//IPR017877 - SANT/Myb domain // Homeodomain-like // Myb-like domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCATAAACTCGTCGCAGTTTGCGTGTCT
Internal bar code:AAGCAGCTCGGCAGGGTAGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:649
LEAP-Seq percent confirming:99.6568
LEAP-Seq n confirming:4646
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTTCGTTCACAACTCCA
Suggested primer 2:AACGTGATTTCCTCGGACAG