| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.161262 |
| Chromosome: | chromosome 6 |
| Location: | 1379852 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258733 | LEU2 | (1 of 1) 2.3.3.13 - 2-isopropylmalate synthase / Isopropylmalate synthetase; Isopropylmalate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCTCCGCGTGTCGGTTTGTTACGTATG |
| Internal bar code: | CCCCCTTACGCGTGGGATCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 97.2973 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGCTCAGAAGGGAACTT |
| Suggested primer 2: | CTGGATGAGTGTGGCTCAGA |