| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161267 |
| Chromosome: | chromosome_16 |
| Location: | 3105153 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g665450 | FAP48,RYR1 | Ryanodine-inositol 1,4,5-triphosphate receptor Ca2+ channel | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCGGTCGCAAATGGAATGAGGCAAGGATT |
| Internal bar code: | TACGCGGTATGTCCACAACTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 958 |
| LEAP-Seq percent confirming: | 97.4359 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGATGTGAGTACGGGGTT |
| Suggested primer 2: | GATTGTGTATGTGGCGCATC |