| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161272 |
| Chromosome: | chromosome 9 |
| Location: | 12363 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386113 | (1 of 2) K01408 - insulysin (IDE, ide) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGCCTGCCTCTCTCGACCCATCTTTTC |
| Internal bar code: | GCGTGTACCCGGAGGTGGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 99.877 |
| LEAP-Seq n confirming: | 812 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGGCAAGTCAAGTAATG |
| Suggested primer 2: | TTGTGGTTCATGTGGCAAGT |