| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.161293 |
| Chromosome: | chromosome 9 |
| Location: | 4072679 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393395 | (1 of 1) PTHR33477:SF3 - EMB|CAB72147.1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTGCACACGCCCCACTCGTGCCTGGAA |
| Internal bar code: | CGGAGGGATGGTCAGCTTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 204 |
| LEAP-Seq percent confirming: | 99.422 |
| LEAP-Seq n confirming: | 688 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACCGTACCAGATCCGAGG |
| Suggested primer 2: | CTGGGCGTTCATACAGGATT |