| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161305 |
| Chromosome: | chromosome 2 |
| Location: | 232836 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074758 | CYG8 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGATGGCATATCTGTGCCGCACAAACA |
| Internal bar code: | GAACGTCGCGCCTGAGATGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 838 |
| LEAP-Seq percent confirming: | 99.7763 |
| LEAP-Seq n confirming: | 446 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCTGTTGCCAAACACTG |
| Suggested primer 2: | TTACACCCATCGCCACTACA |