| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161315 |
| Chromosome: | chromosome 17 |
| Location: | 5905205 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g740050 | (1 of 2) IPR003582//IPR011050//IPR019316 - ShKT domain // Pectin lyase fold/virulence factor // G8 domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGCGCGTGCGTGCAATCTCGCCCATGC |
| Internal bar code: | CGACGTGGCCTCCATGGGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 99.814 |
| LEAP-Seq n confirming: | 1073 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACATCACGTTCACAACCG |
| Suggested primer 2: | TACTGGGAGTTTGAGGTGGG |