| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161323 |
| Chromosome: | chromosome_2 |
| Location: | 5194972 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g105700 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGGACGTGTCTGGCCGCCTGTTCGCGGCG |
| Internal bar code: | GCATTAACCCGGCTGGGACAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 88.3871 |
| LEAP-Seq n confirming: | 959 |
| LEAP-Seq n nonconfirming: | 126 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGACCTGTGCAACCCAA |
| Suggested primer 2: | ACAGTTCACGTGCTGAGTGG |