| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.161336 |
| Chromosome: | chromosome_9 |
| Location: | 1740571 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGGGATGGCAGGTTTGGGATGGCAGGTTT |
| Internal bar code: | GCGTACGAGTAGAACCTACTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 867 |
| LEAP-Seq percent confirming: | 76.4365 |
| LEAP-Seq n confirming: | 1849 |
| LEAP-Seq n nonconfirming: | 570 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAAACTGGGACTCATGT |
| Suggested primer 2: | GCCGCCAAAAATAACAAAAA |