Insertion junction: LMJ.RY0402.161379_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.161379
Chromosome:chromosome 10
Location:2718980
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g438200 (1 of 2) IPR001841//IPR001876//IPR016024 - Zinc finger, RING-type // Zinc finger, RanBP2-type // Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGCGCGCGCCTTTAAGGAGTAGGCAGC
Internal bar code:CCAAGACGTGATCGAGGCGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1046
LEAP-Seq percent confirming:97.4094
LEAP-Seq n confirming:10566
LEAP-Seq n nonconfirming:281
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCCCCATGGCTCTGTTTA
Suggested primer 2:CTGAGTCGCAACAGGTTTGA