| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.161386 |
| Chromosome: | chromosome 5 |
| Location: | 3004295 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238374 | (1 of 1) K11975 - E3 ubiquitin-protein ligase RNF144 [EC:6.3.2.19] (RNF144) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCAGGCGCCGCCGTCGCAGCCCTGCGCC |
| Internal bar code: | CTCTGCTTATCTAGTTATTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 57 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACTGCCGTACACAGGAA |
| Suggested primer 2: | TCTAAGCCTCCTCACCCTGA |