| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161414 |
| Chromosome: | chromosome 6 |
| Location: | 5658751 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286200 | (1 of 4) PTHR22911//PTHR22911:SF6 - ACYL-MALONYL CONDENSING ENZYME-RELATED // SOLUTE CARRIER FAMILY 35 MEMBER G1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTCCGGCTGGCTGCGCCTTAGGCCGCC |
| Internal bar code: | GGCGCCGGGGCTCGGATTGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 99.5955 |
| LEAP-Seq n confirming: | 2216 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTACCTAGCTGCTGATGG |
| Suggested primer 2: | ATGGCTGGTTATAGCAACCG |