| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.161415 |
| Chromosome: | chromosome 3 |
| Location: | 8989938 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g209505 | SNRK2E | snRK1 family in Chlamydomonas, subgroup 2; (1 of 6) K14498 - serine/threonine-protein kinase SRK2 (SNRK2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCGCTGTGCCGTGGAGCTGGACGGCAG |
| Internal bar code: | TTCTTGGAAGTCAGTGTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 4.33071 |
| LEAP-Seq n confirming: | 187 |
| LEAP-Seq n nonconfirming: | 4131 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATCAATCTTGGCAATCT |
| Suggested primer 2: | TCCCTTGTCCTTCCTGACAC |