| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.161427 |
| Chromosome: | chromosome 7 |
| Location: | 3519590 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336100 | (1 of 77) IPR029071 - Ubiquitin-related domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTCCTCGCTCGCCGCTCATCCTGCCCG |
| Internal bar code: | GCGACAAGCGCGATATGTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 94.6128 |
| LEAP-Seq n confirming: | 562 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCTTTTCCTCTGCGTAT |
| Suggested primer 2: | CTCTCGAGTAAGCCCATTGC |