| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161442 |
| Chromosome: | chromosome 12 |
| Location: | 4442177 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521300 | POB14 | Proteome of basal body 14 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGTTTAGAAAGGAGTGTCAAGCTTGCT |
| Internal bar code: | TTAAAACACGCAGAGCCATCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 99.7239 |
| LEAP-Seq n confirming: | 2528 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACATAACAAGCATCACG |
| Suggested primer 2: | CATGCGCAACTAAGCTACCA |