| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161497 |
| Chromosome: | chromosome 4 |
| Location: | 131807 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216811 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGTAATGTGACGGGTTGACTGACTGACT |
| Internal bar code: | GTTTCATCTGATTTGCAGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 924 |
| LEAP-Seq percent confirming: | 99.7191 |
| LEAP-Seq n confirming: | 14910 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCAATCCTTTACCGTTC |
| Suggested primer 2: | GTCTTCAACGGCCATGATCT |