| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161500 |
| Chromosome: | scaffold 24 |
| Location: | 101315 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755847 | (1 of 1) PTHR11935//PTHR11935:SF104 - BETA LACTAMASE DOMAIN // METALLO-BETA-LACTAMASE FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGGAGAAAATGCGCACGGCAAACAGGC |
| Internal bar code: | GTCAAGCCCAAGATGTGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 82.1086 |
| LEAP-Seq n confirming: | 1285 |
| LEAP-Seq n nonconfirming: | 280 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCATTCTTCCTGGAACGG |
| Suggested primer 2: | AGGTTCTCCATCCACGTCAC |