| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161508 |
| Chromosome: | chromosome 6 |
| Location: | 5696032 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286600 | (1 of 1) PF13898 - Domain of unknown function (DUF4205) (DUF4205) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTTGCTCTCTCTTCTGTGTTTCCTTCC |
| Internal bar code: | ACGAGCGTTTCATTTTTCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 47 |
| LEAP-Seq percent confirming: | 99.0099 |
| LEAP-Seq n confirming: | 400 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCAGTCGATGATGCTCAC |
| Suggested primer 2: | CATATCATTACTGCGGTGCG |