| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161509 |
| Chromosome: | chromosome 12 |
| Location: | 7847029 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553050 | NUP53 | (1 of 1) K14313 - nuclear pore complex protein Nup53 (NUP35, NUP53); Nucleoporin 53 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGAAACCCGCGTGCCTCAACGCTTCTGC |
| Internal bar code: | TATGGGTATCCTCCTAAGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 99.5895 |
| LEAP-Seq n confirming: | 2426 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCTATCGGGGAAGGAC |
| Suggested primer 2: | AGGTGTAGACTAGGGGCGGT |