| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161511 |
| Chromosome: | chromosome 3 |
| Location: | 513981 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145247 | TEF11 | S-isoprenylcysteine O-methyltransferase; (1 of 3) 2.1.1.100 - Protein-S-isoprenylcysteine O-methyltransferase / Prenylcysteine carboxyl methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGCAGCTACGACTTACCCAGCAAATTC |
| Internal bar code: | GGAACGGGGCACAACTCGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1030 |
| LEAP-Seq percent confirming: | 99.763 |
| LEAP-Seq n confirming: | 3367 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCCCAACATGTCGAAAC |
| Suggested primer 2: | TAACGCAAGCGAGTAACCCT |