| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161540 |
| Chromosome: | chromosome_3 |
| Location: | 5012793 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g180550 | NTD5,FPN2 | 5\'-nucleotidase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGTTGACCGCATACCAGTTCCGTGCAGCG |
| Internal bar code: | GGGGGGTGCGGAAGTGGGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 343 |
| LEAP-Seq percent confirming: | 97.8012 |
| LEAP-Seq n confirming: | 2135 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCGTTGTCACATACCAG |
| Suggested primer 2: | GACACGTAGTGGTGGTCGTG |