| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.161561 |
| Chromosome: | chromosome 16 |
| Location: | 6721212 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672385 | HIS5 | (1 of 1) 2.6.1.9 - Histidinol-phosphate transaminase / Imidazolylacetolphosphate aminotransferase; Histidinol phosphate aminotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGACGCACACGTCATTCCGTGCGACCT |
| Internal bar code: | GGGCTCGCTGTTGTGTGTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 99.5428 |
| LEAP-Seq n confirming: | 5443 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAACTTGCGGGGTCCTCT |
| Suggested primer 2: | CTGGGTGGTTGTGACTTGTG |