Insertion junction: LMJ.RY0402.161566_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.161566
Chromosome:chromosome_12
Location:7518694
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g555950 DHC11 Putative flagellar inner arm dynein heavy chain, novel minor typ sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GTTTCCTGGTGCGTATGTGTTTGGAAGGCA
Internal bar code:AGAACTTCTCCCGAGGGTTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:589
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1022
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTCTGTTTGTTTCCTTGC
Suggested primer 2:CAGGTTCGTGGGACAAGTTT