| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.161569 |
| Chromosome: | chromosome 3 |
| Location: | 7228870 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207750 | (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCTCGGCGCTGCCATGTTGACAGCGCGG |
| Internal bar code: | CAGTCAGGAAAAATCCGTGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1010 |
| LEAP-Seq percent confirming: | 82.5036 |
| LEAP-Seq n confirming: | 5187 |
| LEAP-Seq n nonconfirming: | 1100 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGCAGGATAACACTGG |
| Suggested primer 2: | TGGTGTTGTTGGGGTATGAA |