Insertion junction: LMJ.RY0402.161579_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.161579
Chromosome:chromosome 11
Location:3776562
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g483200 (1 of 2) PTHR31350//PTHR31350:SF1 - FAMILY NOT NAMED // F-BOX ONLY PROTEIN 21 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGGCCCGCCCCCGGCCCGAACCCCGAAC
Internal bar code:ATACTCGCTTGTTTGCGCCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:863
LEAP-Seq percent confirming:73.2426
LEAP-Seq n confirming:1292
LEAP-Seq n nonconfirming:472
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGATCTACAAGGGGGCAA
Suggested primer 2:AACTGGGTGGAACTTGATGC