| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.161599 |
| Chromosome: | chromosome 16 |
| Location: | 6930891 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676869 | (1 of 10) PTHR23257//PTHR23257:SF520 - SERINE-THREONINE PROTEIN KINASE // IP11267P | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAAAGCGGCTCTCGGCACTTGCGGGCCG |
| Internal bar code: | GAAAAAGTTCCTCCCTTGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 701 |
| LEAP-Seq percent confirming: | 99.4242 |
| LEAP-Seq n confirming: | 10705 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACCGCACATAATTCAG |
| Suggested primer 2: | CTTCTGTCGCATTGGTGGT |