| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.161640 |
| Chromosome: | chromosome 3 |
| Location: | 2808084 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162300 | SDR7 | Short-chain dehydrogenase/reductase; (1 of 13) 1.1.1.300 - NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGTTGCTGTTGAGCCAGTTGGGTCCGA |
| Internal bar code: | GGTTTGGCGGGTGCAAATGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 43 |
| LEAP-Seq percent confirming: | 77.0492 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTGTGAGGAAAGAGGGT |
| Suggested primer 2: | ACAGCCCTTTTGCTTCTTCA |