| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.161641 |
| Chromosome: | chromosome 6 |
| Location: | 3458034 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278094 | ELG14 | Exostosin-like glycosyltransferase 14; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACGTTAGGAGTAGCGGGCTGCTGGGTGC |
| Internal bar code: | GCCCCTTCATTGGTTTTCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1128 |
| LEAP-Seq percent confirming: | 98.8956 |
| LEAP-Seq n confirming: | 2776 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGGAAAGCACCCAAGAG |
| Suggested primer 2: | CCACAATCTCCATTTTGCCT |