| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161647 |
| Chromosome: | chromosome 14 |
| Location: | 1237702 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616350 | FAS4 | Fasciclin-like protein 4; (1 of 10) PTHR10900 - PERIOSTIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAGCGGGTGCACCGGCAGCAGTGGGAGC |
| Internal bar code: | AGCTCCTTGTTGGCAACGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.8626 |
| LEAP-Seq n confirming: | 6542 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGGCCAGTACCTCACT |
| Suggested primer 2: | TTTCGTCTCTCATCACGCAC |