Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.161651 |
Chromosome: | chromosome 11 |
Location: | 1162687 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467687 | (1 of 1) 1.1.1.348 - Vestitone reductase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCTAAACCAACGAATGGGTGGAGAGCA |
Internal bar code: | CTGAACGAGCCGATGTCAATGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 348 |
LEAP-Seq percent confirming: | 99.7956 |
LEAP-Seq n confirming: | 4395 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACCAGCCCAAACTAAACCG |
Suggested primer 2: | ATGCGAATGTGTATGCGTGT |