| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.161658 |
| Chromosome: | chromosome 11 |
| Location: | 3690279 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482650 | EZY3 | Early zygote expressed protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTTGTTCGAGGCAAATTTTTAGGAACC |
| Internal bar code: | GTCCGCTCACTAAAGGGGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 914 |
| LEAP-Seq percent confirming: | 99.6663 |
| LEAP-Seq n confirming: | 896 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCGAAGACCCCCACTAC |
| Suggested primer 2: | CCTGTCAAGGATGCAAGGAT |