| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161658 |
| Chromosome: | chromosome 11 |
| Location: | 3690312 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482650 | EZY3 | Early zygote expressed protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACAACCCGGCGACTGCCAGTGGTGGCTG |
| Internal bar code: | GTGCACATCGGACCAGTCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 614 |
| LEAP-Seq percent confirming: | 99.6721 |
| LEAP-Seq n confirming: | 1520 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTCAAGGATGCAAGGAT |
| Suggested primer 2: | TGTTCGAAGACCCCCACTAC |