| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161664 |
| Chromosome: | chromosome 3 |
| Location: | 851932 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146527 | GSD1 | (1 of 1) 1.2.1.41//2.7.2.11 - Glutamate-5-semialdehyde dehydrogenase / Glutamyl-gamma-semialdehyde dehydrogenase // Glutamate 5-kinase / Gamma-glutamyl kinase; gamma-glutamyl phosphate reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTCTCACCCTCCACGCGCAACTCCTCT |
| Internal bar code: | CGATTAACAGATGGCTTTTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 181 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACTCCTCACTCCCACC |
| Suggested primer 2: | ATGCACCAAAGGGACTTACG |