| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161683 |
| Chromosome: | chromosome 3 |
| Location: | 4480494 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTATACGGCACCGTGCTACCGGCTGAATG |
| Internal bar code: | GGGTTGATGCAGTCACGAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 99.0841 |
| LEAP-Seq n confirming: | 1731 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAAACACAAAGCACCTG |
| Suggested primer 2: | AAAAGCCATTCACAAGGTGG |