| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.161702 |
| Chromosome: | chromosome 10 |
| Location: | 3068662 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441550 | MAM3B | (1 of 5) K16302 - metal transporter CNNM (CNNM); Protein putatively involved in mitochondrial biogenesis | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCACTAGACATTAGGGGACGTGGCAG |
| Internal bar code: | TGCAGGAGGGGGACATCCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1013 |
| LEAP-Seq percent confirming: | 97.6104 |
| LEAP-Seq n confirming: | 5637 |
| LEAP-Seq n nonconfirming: | 138 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGGTGCGTGTATCAGTT |
| Suggested primer 2: | AACCCAACCAAAACCTACCC |