| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.161730 |
| Chromosome: | chromosome_1 |
| Location: | 1650978 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g008650 | CDK1 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGGTGAGGTGCAGGATGGCCGGAAGCTGT |
| Internal bar code: | CTTGGTTGCGGTCAAGAAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 888 |
| LEAP-Seq n nonconfirming: | 148 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCCTGTCTTCCACAACA |
| Suggested primer 2: | GAGCACGGCTACAGAAGACC |