Insertion junction: LMJ.RY0402.161743_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.161743
Chromosome:chromosome 9
Location:450303
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g404450 MCP24 Mitochondrial substrate carrier protein; (1 of 1) IPR000104//IPR002067//IPR018108//IPR023395 - Antifreeze protein, type I // Mitochondrial carrier protein // Mitochondrial substrate/solute carrier // Mitochondrial carrier domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTGCTAATGGTGCTTGCGTGTGTCAGTG
Internal bar code:GGTTCCTCGTCCGTAGGCGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:220
LEAP-Seq percent confirming:93.2013
LEAP-Seq n confirming:1741
LEAP-Seq n nonconfirming:127
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGTCTCAGATAGACACCA
Suggested primer 2:GACAGCGCTTACAATCGACA