Insertion junction: LMJ.RY0402.161779_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.161779
Chromosome:chromosome 9
Location:1240382
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g399550 (1 of 1) PTHR10857//PTHR10857:SF11 - COPINE // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGCAGTCAGTGTGGGTGTGCAGGTATC
Internal bar code:CGGAATGGCTGGGTGATTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:382
LEAP-Seq percent confirming:98.0392
LEAP-Seq n confirming:300
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGCGAAGTTGTAGCCAGG
Suggested primer 2:CTCGGAGCAGTCTCGTTACC