Insertion junction: LMJ.RY0402.161789_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.161789
Chromosome:chromosome_8
Location:4151437
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre08.g379900 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GCCGTACGCTCGTCACCGTACGACAGAGAT
Internal bar code:GCAGGGATTCCCGTAGAGAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1023
LEAP-Seq percent confirming:99.718
LEAP-Seq n confirming:1061
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTCCTGTGCTTTCCTGCT
Suggested primer 2:TTGTAACGACAGCTTGCGAC