| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.161791 |
| Chromosome: | chromosome 2 |
| Location: | 1555189 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084700 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGCCAGCAGGCCCCGGTGGTCTCATAG |
| Internal bar code: | AAAGCGTTTTATTTCTAGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 99.6946 |
| LEAP-Seq n confirming: | 7182 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTGAAATCAGACAACGC |
| Suggested primer 2: | CCTCTGGGTCACAACTCCAT |