| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161819 |
| Chromosome: | chromosome 13 |
| Location: | 3045679 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584450 | (1 of 6) PTHR27000:SF160 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGGCGCCGCAAACGCCCGTCCCCTCC |
| Internal bar code: | GTAGAGGTCTGATAAACGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 97.2588 |
| LEAP-Seq n confirming: | 1774 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGCTGACCACACTCAGA |
| Suggested primer 2: | AAACAGCAGCATCACACAGG |