Insertion junction: LMJ.RY0402.161834_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.161834
Chromosome:chromosome 8
Location:250389
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g358554 (1 of 14) 3.1.3.16//3.1.3.48 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase // Protein-tyrosine-phosphatase / PTPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTAGCGTGCCACCTTACGAGACAGAGATA
Internal bar code:AAGATAGGGGGGGGTGACTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:619
LEAP-Seq percent confirming:94.3662
LEAP-Seq n confirming:134
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:AGATCATCGAAACCCTGCAC
Suggested primer 2:GTGTGTGCATCATGAAAGGG